Sequence of A Peptide

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This kit will illustrate the techniques employed by Frederick Sanger, whose methods won him two Nobel Prizes for primary structure work in protein and nucleic acid chemistry. The experiments in this kit will enable the students to use techniques for determining amino acid content and composition, and identifying N-terminal and C-terminal residue. In carrying out these experiments, the student will determine the amino acid sequence of a tripeptide isolated from a protein hydrosate. The kit provides the means for the identification of the tripetide, ArginylGlyclPhenylalanine. Grades 10-12.